Advanced Plate Sequencing with the BioStack

Read About the Nth Plate step, if you haven't already done so.

To precisely time the events in a BioStack run, consider using the Delay option, Timer Activated Delay.

The LHC makes the BioStack even more powerful by giving you the ability to perform varied or special processes to certain plates or at certain times within the same run.

Numerous scenarios can be served using the LHC's special features, here are a few examples:

• Use an Nth Plate step to flush the tubing or perform a maintenance task after a certain number of plates have been processed.
• Use a series of Nth Plate or Use BioStack steps to test the number of wash cycles required for optimal assay performance.
• Use a series of Nth Plate or multiple Use BioStack steps to perform a serial dilution on recurrent groups of plates: dispense 200 µL to the first plate, 150 µL to the second, and 100 µL to the third.

The Nth Plate lets you create a mixed group of plates that have been processed with different parameters. The Use BioStack step creates groups of like-processed plates. For example, using three Nth Plate steps you can process a stack of plates to stagger the dilutions, as shown in the table below. Similarly, three Use BioStack steps can result in a stack of plates sorted into dilution groups.Learn more...

Using a series of Nth Plate or Use BioStack blocks we can dispense 100 µL to plates in the first pass, 200 µL to plates in the second pass, 300 µL in the third pass. Choose the method that matches your preference for sorting the plates in the stack:

Group	Plate	Nth Plate	Use BioStack
A	1	100 µL	100 µL
	2	200 µL	100 µL
	3	300 µL	100 µL
B	4	100 µL	200 µL
	5	200 µL	200 µL
	6	300 µL	200 µL
C	7	100 µL	300 µL
	8	200 µL	300 µL
	9	300 µL	300 µL

Review Examples:

• Use an Nth Plate step to perform maintenance
• Use a series of Nth Plate steps
• Use a series of Use BioStack steps