LHC
-LHC Software Error Codes
Generally, these errors are caused by protocol parameters that conflict with the instrument's onboard settings. The protocol may have been originally created for a different hardware configuration, the 192-tube wash manifold instead of the 96-tube, for example.
Quick Fix: Make sure your Instrument Settings accurately reflect your instrument’s hardware configuration and then, modify the protocol to fix any invalid parameters. With the instrument connected to your computer and its home/main menu displayed:
- Click the LHC's Settings link in the main view.
- In the Target Instrument Settings dialog, click the instrument link to get the settings from the instrument.
- Modify the protocol step that generated the error message.
|
Error Code |
Description |
Help |
|---|---|---|
|
6000 |
General communication error during download. |
|
|
6001 |
COM port created by USB converter no longer active. |
|
|
6002 |
Invalid basecode part number; instrument is not LHC. |
Service Only. Contact Technical Support. |
|
6003 |
Invalid Basecode Data Version; basecode needs to be updated. |
Contact Agilent to obtain latest basecode. |
|
6004 |
No rows are selected for the specified plate type. |
Modify the protocol to select a row. |
|
6005 |
Invalid row selection value (must be 0 or 1). |
|
|
6006 |
This instrument can only process 96-well plates. |
The protocol may have been created for another instrument, change the plate type or select another protocol.
|
|
6007 |
This instrument can only process 1536-well plates. |
|
|
6008 |
The 8-tube Syringe Manifold can only be used with 96-well plates. |
Mismatch between installed hardware and protocol parameters: change the plate type or correct the instrument settings to match the currently installed hardware.
|
|
6009 |
The 96-tube single wash manifold can only be used with 96-well plates. |
|
|
6010 |
The data is invalid or out-of-range. |
Service
Only. |
|
6011 |
This step type cannot be downloaded. |
Review the limitations to transferring protocols to the instrument, See the LHC Help Topic: Transferring Protocols. |
|
6012 |
Illegal characters in protocol name. |
See the LHC Help topic: Define a Protocol. |
|
6013 |
The protocol name length must be 16 characters or less. |
|
|
6014 |
A 1536 well plate is not supported by this instrument. |
Service
Only. |
|
6015 |
The specified volume exceeds the cassette maximum limit. |
Modify the volume or change the cassette type. |
|
6016 |
The volume is out-of-range. |
Modify the volume or change the cassette type. |
|
6017 |
Invalid flow rate. |
Learn about the Syringe Dispense Step. |
|
6018 |
Invalid
number of |
Service
Only. These codes indicate an unexpected software error that cannot be fixed without Agilent support.
|
|
6019 |
Invalid horizontal dispense position. |
|
|
6020 |
Invalid dispense height. |
|
|
6021 |
Invalid plate clear height. |
|
|
6022 |
Invalid column selection value (must be 0 or 1). |
|
|
6023 |
Invalid protocol step type. |
|
|
6024 |
The Definition String contains invalid data. |
|
|
6025 |
Manifold conflict between protocol requirements and instrument configuration. |
Change
the Washer Manifold or change the Instrument Setting. |
|
6026 |
Valve module conflict between protocol requirements and instrument configuration. |
Make sure the Buffer Switching setting matches your instrument; see Instrument Settings. |
|
6027 |
Syringe module conflict between protocol requirements and instrument configuration. |
Make sure the Syringe dispenser setting matches your instrument: see Instrument Settings. |
|
6028 |
Vacuum filtration washer conflict between protocol requirements and instrument configuration. |
The vacuum filtration carrier must be installed. Be sure the plate carrier setting is correct. Otherwise, contact Technical Support. |
|
6029 |
Required cassette does not match installed cassette. Using RAD technology cassettes increases the chance of getting this error. Be as precise as possible when using this feature. |
Change the cassette type to match the protocol requirement and rerun the protocol. |
|
6030 |
Invalid cassette type was specified. |
Service
Only. |
|
6031 |
Cannot use a 96-well plate with a 192-tube manifold. |
Modify the Plate Type or Change the Washer Manifold. |
|
6032 |
Downloading Protocols is not supported. |
Service
Only. |
|
6033 |
This step is not supported for 1536-well plates. |
Fix the plate type or the Instrument Settings. A conflict between the plate type and installed hardware devices has been detected. Change the Plate Type to one supported by the washer/dispenser. Or, click the Instrument Settings link and make sure they match the physical hardware: Get settings from instrument. |
|
6034 |
The 32-tube Syringe Manifold is required for 1536-well plates. |
|
|
6035 |
The 16-tube Syringe Manifold is required for 96- and 384-well plates. |
|
|
6036 |
The 128-tube Washer Manifold is required for 1536-well plates. |
|
|
6037 |
The 128-tube Washer Manifold can only be used for 1536-well plates. |
|
|
6038 |
This step only applies to 1536-well plates. |
|
|
6039 |
Conflicting column selection. |
Fix the plate map (selected columns to dispense to). A protocol parameter may have been changed after a partial plate dispense was defined. |
|
6040 |
Invalid baud rate |
Service
Only. These codes indicate an unexpected software error that cannot be fixed without Agilent support.
|
|
6041 |
Invalid data bits selection |
|
|
6042 |
Invalid stop bits selection |
|
|
6043 |
Invalid parity selection |
|
|
6044 |
Serial port error |
Fix the COM port setting. Check the cabling. Click the Port link and use the drop-down menu to see all active ports. Customize the Predefined Protocols to avoid this error in future. When controlling the BioStack with the LHC, make sure the instrument's BioStack setting is Manual. |
|
6045 |
Serial write error |
|
|
6046 |
Serial read error |
|
|
6047 |
Checksum error |
Contact Technical Support. |
|
6048 |
Serial NAK error |
Make sure the COM port setting is correct and the cable is properly connected. Restart the instrument. If error reoccurs: Contact Technical Support. |
|
6049 |
Excess data, or not enough data, received. |
To correct these errors:
If error reoccurs, contact Technical Support. |
|
6050 |
Invalid message header |
|
|
6051 |
Invalid message object |
|
|
6052 |
Invalid message body size |
|
|
6053 |
Serial message timeout |
|
|
6054 |
Port handle error |
|
|
6055 |
Read timeout value is invalid. |
|
|
6056 |
Unauthorized to open the COM port. |
Make sure the COM port setting is correct and the cable is properly connected. Restart the instrument. If error reoccurs:Contact Technical Support. |
|
6057 |
Out-of-range parameter for the open port function. |
|
|
6058 |
Unable to open the COM port. |
|
|
6059 |
Unable to clear the transmission buffer. |
|
|
6060 |
Unable to close the port. |
|
|
6061 |
Port is no longer available. |
|
|
6062 |
Unhandled exception while transmitting message. |
Contact Technical Support |
|
6063 |
The selected plate type is not allowed with this protocol step. |
Modify the protocol to change the plate type. |
|
6064 |
The protocol specifies more Peri-pumps than are available. |
The protocol may have been created for a different instrument. Make sure the instrument settings match the current instrument and modify the protocol. |
|
6065 |
Too few data bytes received from the instrument. |
|
|
6066 |
Ultrasonic cleaning assembly is not installed. |
The protocol may have been created for a different instrument. Make sure the instrument settings match the current instrument and modify the protocol. |
|
6067 |
The type of Syringe pump is not compatible with the syringe manifold. |
|
|
6070 |
Invalid Syringe specified. |
Service
Only.
These codes indicate an unexpected software error that cannot be fixed without Agilent support.
|
|
6071 |
Invalid number of syringe prime cycles. |
|
|
6072 |
Invalid syringe Aspirate Delay value. |
|
|
6073 |
Invalid X-axis offset value. |
|
|
6074 |
Invalid Y-axis offset value |
Service
Only. These codes indicate an unexpected software error that cannot be fixed without Agilent support.
|
|
6075 |
Invalid Z-axis offset value |
|
|
6076 |
Vacuum filtration not allowed with 1536-well plates. |
Modify the protocol to change the plate type. |
|
6080 |
Invalid Peri-pump prime duration |
Service
Only.
These codes indicate an unexpected software error that cannot be fixed without Agilent support. |
|
6085 |
Invalid minutes:seconds value |
|
|
6086 |
Invalid hours:minutes value |
|
|
6087 |
'Move carrier home' is required when duration exceeds 1 minute. |
Contact Technical Support |
|
6088 |
Invalid Shake/Soak options selected |
Service
Only.
These codes indicate an unexpected software error that cannot be fixed without technical support. |
|
6089 |
Invalid Shake Intensity selected |
|
|
6090 |
Invalid Washer buffer selected |
|
|
6091 |
Invalid Washer Aspirate Delay value |
|
|
6092 |
Invalid Washer Aspirate Travel Rate value |
|
|
6093 |
Invalid Wash Cycles value |
|
|
6094 |
Invalid Wash format selected |
|
|
6095 |
Invalid Wash Sectors selected |
|
|
6096 |
Wash Aspirate Delay value is required. |
|
|
6097 |
Syringe Dispense Volume must be an integer. |
|
|
6098 |
Peri-pump cannot run with the pump cover open. |
Close the pump cover door and rerun the protocol. |
|
6099 |
Peri-pump assembly not installed. |
Physically install the Peri-pump and/or make sure the instrument settings reflect the current state. |
|
6100 |
This functionality requires the software to be registered. |
You must register the software with BioTek. Select Help>Register Software. |
|
6101 |
Basecode download is not allowed for this instrument |
Service
Only. |
|
6102 |
The "Mini-Tube" plate type must be used with the "Mini-Tube" plate carrier. |
Verify the Plate Carrier instrument setting is accurate and the protocol's "Plate Type" is supported by the carrier. |
|
6110 |
Verify Technology test parameters file could not be found. |
Service
Only. |
|
6111 |
Error reading Verify Technology test settings file. |
Service
Only. |
|
6112 |
Verify Technology test was canceled. |
User canceled operation; whether this was intentional or not, you must restart the test. |
|
6113 |
Verify Technology not supported. |
Service
Only. |
|
6120 |
Verify Technology test plate not level in the carrier. |
Review guidelines: Verify Technology Level Sensor Troubleshooting.
|
|
6121 |
Verify Technology test encountered an aspirate scan error. |
|
|
6122 |
Verify Technology test encountered a dispense scan error. |
|
|
6123 |
Center of well not found where expected for Verify Test Plate. |
Review guidelines: Verify Technology Level Sensor Troubleshooting. |
|
6124 |
Incorrect plate used for Verify Technology test. |
You must use the calibrated Verify Test Plate to perform the test. |
|
6125 |
The well volume following an aspirate step indicates insufficient aspiration. |
Aspirate tubes appear to be clogged. Clean the manifold and restart the test. |
|
6126 |
The well volume following a dispense step indicates insufficient dispensing. |
Dispense tubes appear to be clogged. Clean the manifold and restart the test. |
|
6127 |
Verify Technology test data could not be obtained from the instrument. |
Service
Only. |
|
6128 |
During Verify Technology test, an invalid well was specified. |
Service
Only. |
|
6129 |
Verify Technology test was not performed because of an error. |
One of the previous Verify tests failed - there are five tests in total. Take action to resolve the failure and restart the test. See About Verify™ Technology. |
|
6150 |
The mean dispense volume is out of the acceptable range. |
Dispense tubes appear to be clogged. Clean the manifold and restart the test. See Fluid Delivery . |
|
6151 |
The dispense %CV exceeds the maximum threshold. |
|
|
6152 |
The aspirate rate is below the minimum threshold. |
Aspirate tubes appear to be clogged. Clean the manifold and restart the test. Fluid Aspiration . |
|
6160 |
Strip washer required to perform this step, but not installed. |
Make sure the protocol you are trying to run is supported by your instrument's devices. |
|
6161 6162 |
Strip washer manifold and plate type are incompatible. Strip washer doesn't support this plate type. |
Make sure the specified plate type matches the installed manifold and the instrument's manifold setting correctly identifies the installed manifold. |
|
6165 |
This Peri-pump does not support single well dispensing. |
An external Peri-pump is required for RAD technology cassettes. Edit the protocol to use the correct Peri-pump, i.e. protocols designed for a Primary pump cannot be run on a Secondary pump. |
|
6166 |
This instrument does not support RAD technology dispensing. |
Make sure the protocol you are trying to run is supported by your instrument's devices. |
|
6167-6170 |
The Syringe manifold does not support the plate type. |
Make sure the specified plate type matches the installed manifold and the instrument's manifold setting correctly identifies the installed manifold. |
|
6171 |
The installed RAD cassette does not support this plate type. |
Either use a supported plate type, correct the instrument's cassette type setting or edit the protocol. |
|
6200 |
Invalid wash manifold for specified plate type. |
Either use a supported plate type, correct the instrument's "Plate Carrier" setting, or edit the protocol. |
|
6201 |
"Prompt" step text is too long. |
Service
Only. |